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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » Hyaluronidase, Bovine

Hyaluronidase, Bovine

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Specifications

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-beta-D-glucose and D-glucose residues in hyaluronate. Hyaluronate 4-glycanohydrolase (EC 3.2.1.35) catalyzes the depolymerization of mucopolysaccharides, hyaluronic acid, and the chondroitin sulfates A and C. The enzyme is widely distributed in animal tissues but is found in great concentrations in the bovine and ovine testes. It is also produced by a number of bacteria. Hyaluronidase from bovine testes has a molecular weight of 55,000. Purified hyaluronidase is used clinically for the intradermal administration of large volumes of fluid when intravenous injections are contraindicated. The enzyme is administered prior to or simultaneously with the fluid and it facilitates absorption of the fluid.
Catalog #H7981-01
SourceBovine testes
SolubilitySoluble in ddH2O or dilute buffer.
Protein Content~90% (biuret)
Activity 300u/mg
Unit DefinitionThat amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37°C and pH 4.0. The assay is based on the following reaction:
Hyaluronic acid ------> Acetylglucosamine
An anhydro sugar is first formed from N-acetylglucosamine, followed by the conversion of the sugar into its furan derivative, as a result of increasing the acidity of the solution. This furan derivative then reacts with p-dimethylamino-benzaldehyde to form a colored complex, which is measured spectrophotometrically at 585nm.
Quality ControlSDS-PAGE
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number37326-33-3
Form
Supplied as a dialyzed, lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBovine testes, Hyaluronate 4-glycanohydrolase, EC=3.2.1.35


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