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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -HRAS, Palmitoylated (GTPase HRas, Transforming protein p21, p21ras, H-Ras-1, c-H-ras, Ha-Ras, HRAS1)

Anti -HRAS, Palmitoylated (GTPase HRas, Transforming protein p21, p21ras, H-Ras-1,
c-H-ras, Ha-Ras, HRAS1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Ras proteins function as GDP/GTP-regulated binary switches in signal transduction cascades that can lead to cell growth, proliferation, differentiation, or survival. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. This Ras superfamily has at least five major branches that include Ras, Rho, Ran, Arf/Sar, Rab. Ras itself has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. They are collectively referred to as Ras. In its active form, Ras is bound to GTP. This causes a conformational change that allow it to interact and bind to several effector molecules, most notably the members of the Raf family, the RalGDS family, and Phosphoinositide 3-kinases (PI3 Kinase). Ras then cleaves GTP to GDP resulting in its inactivation. In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways.
Catalog #H8010-02
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic linear peptide corresponding to the hypervariable region of Ras, H, palmitoylated (KLH).
SpecificityRecognizes the hypervariable region of human Ras, H Palmitoylated. Species sequence homology: Rat, Mouse, Chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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