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You are here:Home » Antibodies » Antibodies-Nucleotides » Anti -8-Hydroxyguanosine (8-OHG)

Anti -8-Hydroxyguanosine (8-OHG)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Reactive oxygen species (ROS) are formed at high levels as by-products of the normal cellular metabolism. Both nuclear and mitochondrial DNA has been shown to accumulate high levels of 8-hydroxy-2'-deoxyguanosine, a very stable and damaging product of hydroxylation of guanine at carbon 8. 8-hydroxyguanosine (8-OHG) induces transversion of G to T, which is potentially mutagenic. The base excision repair (BER) pathway is the most important cellular protection mechanism responding to oxidative DNA damage. They remove modified DNA bases before they are incorporated into DNA during replication. The key enzymes MutT homologs (MutT/MTH) in the BER process are DNA glycosylases, which remove different damaged bases by cleavage of the N-glycosylic bonds between the bases and the deoxyribose moieties of the nucleotide residues. The 8-oxoG glycosylases (Fpg or MutM/OGG) and the MutY homologs (MutY/MYH) glycosylases along with MutT/MTH protect cells from the mutagenic effects of 8-oxoG. 8-hydroxyguanosine (8-OHG) has been used as oxidative stress marker.
Catalog #H9076-01A
ApplicationsSuitable for use in ELISA and Immunohistochemistry (PLP-fixed sections). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9L175
FormSupplied as a liquid in TBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
Immunogen8-hydroxyguanosine (oh8G)-BSA and –casein conjugates.
SpecificityRecognizes 8-Hydroxyguanosine. Crossreactivity: 8-Hydroxy-2’-deoxyguanosine , 8-Hydroxyguanine and 8-Hydroxyguanosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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