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Specifications

Clone Host Grade Applications Polyclonal Goat Serum E IH

8-hydroxyguanosine (8-OG) is a modified base which occurs in DNA as a result of oxidative stress. 8-hydroxy-2'-deoxyguanosine (8-OHdG) has become a sensitive marker of oxidative damage in cellular DNA and has been reported to be excreted in the urine. Levels of 8-OHdG have been shown to increase on exposure to ionizing radiation and tobacco smoke.
Catalog #	H9076-02
Applications	Suitable for use in Immunohistochemistry and ELISA. Other application not tested.
Recommended Dilutions	Immunohistochemistry (Paraffin): 1:200
	ELISA: 1:100,000-1:250,000
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Antibodies	I1904-28C: IgG, H&L (HRP) Pab DkxGt
	I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	Not Determined
Form	Supplied as a liquid, 0.1% sodium azide.
Purity	Serum.
Immunogen	8-Hydroxyguanosine-conjugate.
Specificity	Recognizes 8-hydroxyguanosine (8-OG). Crossreacts completely with 8-OHdG. Does not crossreact with other naturally occurring nucleotides.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.