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H9100 Mouse Anti-3-Hydroxykynurenine (3-OHKYN)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
P3UI
Grade
Affinity Purified
Applications
E IC IF IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

3-hydroxykynurenine, a chromophore metabolite of tryptophan, acts as UV protectant and an antioxidant in vertebrate lens. This compound reacts with proteins to induce cross-linking and chromophore adducts.

Applications
Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot, Other applications not tested.
Recommended Dilution
Immunocytochemistry/Immunofluorescence: 1:100-1:200 Western Blot: 2ug/ml. Band at ~66kD observed for 3-OHKYN-modifed BSA. Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Cellular Localization
Cytoplasmic
Positive Control
3-OHKYN modified BSA
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
3-OHKYN modified KLH
Form
Supplied as a liquidin Tris-glycine, 150mM sodium chloride, 0.05% sodium azide
Purity
Purified by Protein G affinity chromatography
Specificity
Specific for 3-hydroxykynurenine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan. Reacts with 3-OHKYN modifications in human proteins.
References
Staniszewska, MM and Nagaraj, RH. 3-hydroxykynuenine-mediated modification of human lens proteins. JBC. 280 (23): 22154-22164, 2005
USBio References
No references available
Conjugates
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