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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -3-Hydroxykynurenine (3-OHKYN)

Anti -3-Hydroxykynurenine (3-OHKYN)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IC IF
3-hydroxykynurenine, a chromophore metabolite of tryptophan, acts as UV protectant and an antioxidant in vertebrate lens. This compound reacts with proteins to induce cross-linking and chromophore adducts.
Catalog #H9100
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (paraffin) and Western Blot, Other applications not tested.
Recommended DilutionImmunocytochemistry/Immunofluorescence: 1:100-1:200
Western Blot: 2ug/ml. Band at ~66kD observed for 3-OHKYN-modifed BSA.
Optimal dilutions to be determined by the researcher.
Cellular LocalizationCytoplasmic
Positive Control3-OHKYN modified BSA
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No6k292
FormSupplied as a liquidin Tris-glycine, 150mM sodium chloride, 0.05% sodium azide
PurityPurified by Protein G affinity chromatography
Immunogen3-OHKYN modified KLH
SpecificitySpecific for 3-hydroxykynurenine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan. Reacts with 3-OHKYN modifications in human proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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