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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IC

3-hydroxykynurenine, a chromophore metabolite of tryptophan, acts as UV protectant and an antioxidant in vertebrate lens. This compound reacts with proteins to induce cross-linking and chromophore adducts.
Catalog #	H9100
Applications	Suitable for use in ELISA, Western Blotting, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blotting: 2ug/ml. Band is seen at ~66kD
	Optimal dilutions to be determined by the researcher.
Cellular Localization	Cytoplasmic
Positive Control	3-OHKYN modified BSA
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6k292
Host	Mouse
Concentration	~4mg/ml
Form	Ascites, Tris-glycine, 150mM sodium chloride 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	3-OHKYN modified KLH.
Specificity	Specific for 3-hydroxykynureinine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan. Reacts with 3-OHKYN modifications in human proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.