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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH

Catalog #	H9118
Applications	Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
SpecificityUsing a conjugate 5 hydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds
	Compounds Cross-reactivity ratio(a)5 Hydroxytryptamine-G-PL 15 Hydroxytryptamine-G-PL (b) 1/105 Methoxytryptamine-G-PL 1/25Tryptamine-G-PL 1/515 Hydroxytryptophan-G-PL 1/1755 Hydroxytryptamine 1/1,150Tryptophan-G-PL 1/3,4005 Methoxytryptophan-G-PL 1/19,800
(a)	5 Hydroxytryptamine-G-PL concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
	(b) : Non-reduced conjugate
	G = Glutaraldehyde, PL = poly-Lysine
	Recommended dilutionThe antiserum was tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 5-hydroxytryptamine antibodies stains well between a 1/2,000-1/5,000.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity	Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen	5 Hydroxytryptamine Glutaraldehyde Carriers.
Specificity	Recognizes protein-conjugated Hydroxytryptamine,5-. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.