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You are here:Home » Antibodies » Abs to Amino Acids » Anti -5-Hydroxytryptophan, Conjugated

Anti -5-Hydroxytryptophan, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
Catalog #H9119
Application(s) Suitable for use in Immunohistochemistry, ELISA. . Other applications not tested.
Recommended Dilution Immunohistochemistry 1:2000-1:5000. Tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 5-hydroxytryptophan antibodies stains well.
Additional crossreactivity dataUsing a conjugate 5 hydroxytryptophan-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compounds Crossreactivity ratio(a) 5-Hydroxytryptophan-G-BSA 1 5-Methoxytryptophan-G-BSA 12000
Tryptophan-G-BSA 1:50,000
5-Hydroxytryptamine-G-BSA 1:50,000
Tryptamine-G-BSA 1:>50,000
5-Hydroxytryptophan 1:>50,000
(a) 5-Hydroxytryptophan-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement. G = Glutaraldehyde, BSA
PAP procedureSecond antibody : Sections are incubated with 1/100 dilution of goat anti-rabbit in solution C for 3 hours at 20°C or 1 hour at 37°C. Then, they are washed 3 times (10 min) with solution C.
PAP Sections are incubated with 1/1000 dilution of rabbit peroxidase anti-peroxidase complex in solution C for 1 hour at 37°C. Then, they are washed 3 times (10 min) with solution C.
Antibody-antigen complexes are revealed using diaminobenzidine (25mg/100ml) (or other chromogen) dissolved in Tris 0.05M and filtrated ; 0.05% of H2O2 is added. The sections are incubated for 10 min at 20°C. Reaction is stopped by transfering sections in 5ml of Tris 0.05M.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen5 Hydroxytryptophan Glutaraldehyde Carriers.
SpecificityRecognizes protein-conjugated 5-Hydroxytryptophan. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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