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H9700 Hygromycin B 50mg/ml Solution CAS: 31282-04-9

Specifications
References
CAS Number
31282-04-9
Grade
Molecular Biology Grade
Molecular Formula
C20H37N3O13
Molecular Weight
527.5
EU Commodity Code
38220090
UN DOT Shipping
UN2810 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C
Streptomyces hygroscopicus

Hygromycin B is an aminoglycosidic antibiotic which inhibits protein synthesis in bacteria, fungi and mammalian cells and is used to select prokaryotic and eukaryotic cells that have been transfected with the Hygromycin gene (hyg, hph, HMA, HM+).

Source: Streptomyces hygroscopicus
Purity (HPLC): ≥ 76%
Bioassay: 300-500units/mg solution
Density: 1.13g/ml
Concentration: 50mg Hygromycin B/ml
Form: Supplied as a liquid. Prepared with RNase, DNase-free, molecular biology grade water (W0900). Sterile-filtered (0.2um).
Recommended Concentration Range: 25-1500ug/ml E.coli JM 83: 25-200ug/ml Human: 50-400ug/ml Saccharomyces cerevisiae: 200ug/ml Chicken: 1500ug/ml Tobacco: 20-200ug/ml
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
References
Kojic, M. and Holloman, W.K. (2000) Can J Microbiol. 46:333-338. 1. Hamada, W., et al. 1994. Curr. Genetics 26, 251. 2. Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731. 3. Gaken, J., et al. 1992. Biotechniques 13, 32. 4. Ma, H., et al. 1992. Gene 117, 161. 5. Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932. 6. Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53. 7. McIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67. 8. Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
USBio References
US Biological application reference: Kojic, M. and Holloman, W.K. (2000) Can J Microbiol. 46:333-338.
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