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Specifications

Hygromycin B is an aminoglycosidic antibiotic which inhibits protein synthesis in bacteria, fungi and mammalian cells and is used to select prokaryotic and eukaryotic cells that have been transfected with the Hygromycin gene (hyg, hph, HMA, HM+).
Catalog #	H9700
Source	Streptomyces hygroscopicus
Purity (HPLC)	76%
Bioassay	300-500units/mg solution
Density	1.13g/ml
Concentration	50mg Hygromycin B/ml
Form	Supplied as a liquid. Prepared with RNase, DNase-free, molecular biology grade water (W0900). Sterile-filtered (0.2um).
Recommended Concentration Range	25-1500ug/ml
	E.coli JM 83: 25-200ug/ml
	Human: 50-400ug/ml
	Saccharomyces cerevisiae: 200ug/ml
	Chicken: 1500ug/ml
	Tobacco: 20-200ug/ml
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	31282-04-9
Molecular Formula	C20H37N3O13
Molecular Weight	527.5
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Streptomyces hygroscopicus