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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Hypoxia Inducible Factor 1a (HIF-1a)

Anti -Hypoxia Inducible Factor 1a (HIF-1a)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC IF
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Catalog #H9807-01
ApplicationsSuitable for use in Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:500 (ECL)
Immunohistochemistry (Paraffin and frozen): 1:25-1:100
Optimal dilutions to be determined by the researcher.
Positive controlNuclear extracts
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No3C142
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion protein containing aa432-528 of human HIF-1 alpha. Cellular Localization: Nuclear.
SpecificityRecognizes human HIF-1 alpha. Recognizes bands at 120kD representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120kD representing post-translational modification of HIF-1 alpha. Species crossreactivity: monkey, sheep, mouse, rat, ferret, porcine, hamster, bovine and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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