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Specifications

The human cytokine I-309 is a small glycoprotein secreted by activated T lymphocytes and structurally related to a number of inflammatory cytokines. Recombinant Human I-309 is a single,non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8.504kD.
Catalog #	I0100-15
Sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Ser-Met-Gln.
Dimers and Aggregates	1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity	Human I-309 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T cells at 10.0-100.0ng/ml.
Endotoxin	0.1ng/ug (IEU/ug) of Human HCC-1.
Protein Content	Protein quantitation was carried out by two independent methods:
	1. UV spectroscopy at 280nm.
	2. Analysis by RP-HPLC, using a calibrated solution of Human I-309 as a Reference Standard.
Reconstitution	Reconstitute the lyophilized Human I-309 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source	E. coli
Purity	98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder. No additives.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.