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You are here:Home » Antibodies » Antibodies-CD Markers » Anti -ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850)

Anti -ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte
immunomediatory molecule, AILIM, MGC39850)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E FC
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1)/ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain.
Catalog #I0650-01
ApplicationsSuitable for use in ELISA and Flow Cytometry and Functional application. Other applications not tested.
Recommended DilutionFunctional Application: Antibody blocks binding of Recombinant GL50-muIg to ICOS
Flow Cytometry: 1ug/ml. 5x10e5 human HPB-MLT cells were washed, incubated for 45 min. on ice with 80ul of
I0650-01 at 1ug/ml. Cells were washed twice and incubated appropriate secondary antibody, after which they were washed twice, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to isotype control mouse IgG.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H152
FormSupplied as a liquid in PBS, pH 7.5, 0.5 mg/ml Gentamicin Sulfate.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman HPB-MLT cells, recombinant human ICOS-muIg
SpecificityBinds to recombinant and cell surface ICOS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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