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Anti -ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850) (Biotin)
Anti -ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte
immunomediatory molecule, AILIM, MGC39850) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E FC |
|
| The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1). Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS(3). Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. | | | Catalog # | I0650-01A | | Applications | Suitable for use in ELISA, Flow Cytometry and Functional Studies. Other applications not tested. | | Recommended Dilution | Functional Application: Blocks binding of recombinant GL50-muIg to HPB-MLT cells. | | Flow cytometry: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Protocol | 1. Five x 10e5 cultured human HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of I0650-01A at 1ug/ml. | | 2. Cells were washed twice and incubated with Streptavidin(PE) (S7973-88P) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.38 log10 fluorescent units when compared to a Mouse IgG1(Biotin) negative control at a similar concentration. | | 3. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD278 antibody (I0650-01). | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 7H152 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Human HPB-MLT cells, recombinant human ICOS-muIg. | | Specificity | Recognizes human ICOS. Binds to recombinant ICOS-muIg in ELISA, and to the cell surface of HPB-MLT cells in flow cytometry. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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