Anti -ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2,
B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG,
KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B FC N |
|
| The human ICOS Ligand (B7-H2) is a 60kD member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20-25% aa identity. Within the extracellular domain, human B7-H2 shares 49% and 54% aa sequence identity with mouse and rat B7-H2, respectively. The ligand for ICOS is a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 aa protein with a putative 18aa signal peptide, a 239aa extracellular domain, an 18aa transmembrane region and a 33aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation. | | | Catalog # | I0650-01D | | Applications | Suitable for use in Neutralization, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested. | | Recommended Dilutions | Neutralization: Measured by its ability to neutralize B7-H2-induced proliferation in PHA-activated human T cells. The Neutralization Dose (ND50) is typically 1-4ug/ml in the presence of 3ug/ml Recombinant Human B7-H2 Fc Chimera and 20ng/ml Human CD3-epsilon Mab. | | Flow Cytometry: 2.5ug labels 10e6 U937 cells | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 8H104 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS. | | Purity | Purified by Protein A affinity chromatography. | | Endotoxin | 0.1EU/ug (LAL). | | Immunogen | Recombinant corresponding to Asp19-Ser258 from human ICOS Ligand expressed in NSO cells (O75144). | | Specificity | Recognizes human ICOS Ligand (B7-H2). Does not crossreact with recombinant human B7-1, B7-2, B7-H1, B7-H3 or recombinant mouse B7-H2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | B7RP-1, B7h, LICOS, GL50 |
|
|
Questions or comments about this product? Inquire here. |
|
|
Download Specifications for Anti -ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand) |
External Links
|
