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You are here:Home » Antibodies » Abs to Ligands » Anti -ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)

Anti -ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2,
B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG,
KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B FC N
The human ICOS Ligand (B7-H2) is a 60kD member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20-25% aa identity. Within the extracellular domain, human B7-H2 shares 49% and 54% aa sequence identity with mouse and rat B7-H2, respectively. The ligand for ICOS is a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 aa protein with a putative 18aa signal peptide, a 239aa extracellular domain, an 18aa transmembrane region and a 33aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Catalog #I0650-01D
ApplicationsSuitable for use in Neutralization, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionsNeutralization: Measured by its ability to neutralize B7-H2-induced proliferation in PHA-activated human T cells. The Neutralization Dose (ND50) is typically 1-4ug/ml in the presence of 3ug/ml Recombinant Human B7-H2 Fc Chimera and 20ng/ml Human CD3-epsilon Mab.
Flow Cytometry: 2.5ug labels 10e6 U937 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H104
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein A affinity chromatography.
Endotoxin<0.1EU/ug (LAL).
ImmunogenRecombinant protein corresponding to Asp19-Ser258 from human ICOS Ligand expressed in NSO cells (O75144).
SpecificityRecognizes human ICOS Ligand (B7-H2). Does not crossreact with recombinant human B7-1, B7-2, B7-H1, B7-H3 or recombinant mouse B7-H2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesB7RP-1, B7h, LICOS, GL50


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