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You are here:Home » Antibodies » Abs to Ligands » Anti -ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein Gl50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7 H2 ICOS Ligand)

Anti -ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2,
B7-like Protein Gl50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG,
KIAA0653, LICOS, Transmembrane Protein B7 H2 ICOS Ligand)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B N
ICOS Ligand, also known as B7-related protein 1 (B7RP-1), is a costimulatory molecule that serves as a ligand for ICOS (Inducible Co-Stimulator).
Catalog #I0650-01H
ApplicationsSuitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsNeutralization: ~1-4ug/ml yields one-half maximal inhibition (ND50) of the cell surface ICOS Ligand in the presence of 20ng/ml of anti-human CD3 and 3ug/ml of recombinant human B7-H2/Fc, using B7-H2-dependent proliferation of PHA stimulated human T cells as an assay.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well.
Western Blot: 1-2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
HybridomaHybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human ICOS Ligand extracellular domain.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H103
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
Endotoxin0.1EU/ug (LAL).
ImmunogenNS0-derived recombinant human ICOS Ligand extracellular domain.
SpecificityRecognizes human ICOS Ligand. No crossreactivity with recombinant human B7-1, B7-2, B7-H1, B7-H3, recombinant mouse B7-H2 or PD-L2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesB7RP-1, B7h, LICOS, GL50


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