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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IC IF
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
Catalog #I0800-08K
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:20
Western Blot: 1:10, detects a band at ~130kD
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No7C229
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenRecombinant human iNOS.
SpecificityRecognizes human iNOS (aa1009-1026) and recombinant human iNOS (Type II). Does not react to heNOS (aa1040-1057), rheNOS (Type III), hnNOS (aa1280-1297) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153aa length of hiNOS and was found to bind to a defined region of the hiNOS sequence, aa1009-1026.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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