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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
Catalog #I0800-09
NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionELISA: Control peptide should be coated at 1ug/ml.
Western Blot: 1:100-1:1000. Recognizes 130kD protein.
Optimal dilutions to be determined by the researcher.
Control Peptide I0800-09A
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid, serum, 0.1% sodium azide, 40% glycerol.
PuritySerum
ImmunogenSynthetic peptide mapping to 14 amino acids within the C-terminal of mouse nitric oxide synthase (KLH coupled). Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins.
SpecificityRecognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). No significant sequence homology of iNOS-P is seen with NOS-1/NOS-3 or other proteins. Species homology: Human and rat (85%, 12/14 aa).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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