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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH IC IF
Catalog #I0800-10
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000. A single band at ~130kD was detected using homogenate from cells expressing hiNOS.
Indirect Immunofluorescence: 1:1000-1:5000
Immunohistochemistry: Frozen. not recommended for paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile 40-50% glycerol, PBS containing10mg/ml BSA. No preservative.
PuritySerum
ImmunogenHiNOS (Cys1137 - 1138-1153) covalently attached to a carrier protein.
SpecificityRecognizes human Inducible Nitric Oxide Synthase (iNOS). Specific for the carboxyl end of hiNOS.
CrossreactivityHiNOS (1137-1153)
Species crossreactivityDoes not crossreact with rat iNOS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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