Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IH IC |
|
| Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: Nterminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and Cterminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaMbinding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.. | | | Catalog # | I0800-12 | | Applications | Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000 detects INOS on 10ug of mouse liver lysates | | Immunocytochemistry: 1:50,000 (Cultured cells) | | Immunohistochemistry:1:5000 (ABC). Tissue sections of rat liver | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | Not Determined | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide 14aa from C-terminus mouse macrophagae nitric oxide synthase. | | Specificity | Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II) at ~131kD. No crossreactivity with rat nNOS and eNOS by Western Blot and Immunohistochemistry. Species Crossreactivity: rat. Crossreactivity with human iNOS is ~1%. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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