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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IC IF
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and antipathogenic activities. NOS is classified under three types: Type I NOS is 150-160kD and is found in neurons. It is constitutively expressed and is Ca2+/calmodulin dependent. It is called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral/constitutive/Ca2+ regulated NOS (cNOS). bNOS is present in skeletal muscles and its absence in Duchenne’s muscular dystrophy accounts for its symptoms of the disease.
Type II NOS is 130kD protein known as macrophage NOS (mNOS) or inducible NOS (iNOS). It is Ca2+/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. Type III NOS is a 135kD protein called endothelial NOS (eNOS).
Catalog #I0800-14M
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:20
Western Blot: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No7C226
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenRecombinant hiNOS
SpecificityRecognizes hiNOS (781-798) and rhiNOS (Type II). Does not react to heNOS (806-823), rheNOS (Type III), hnNOS (1045-1062) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 781-798.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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