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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IC IF
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
Catalog #I0800-14T
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:500
Western Blot: 1:1000. A single band is detected at ~130kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No7C204
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PurityAscites
ImmunogenRecombinant hiNOS
SpecificityRecognizes hiNOS (25-54) and rhiNOS (Type II). Does not react to hnNOS (201-231), rhnNOS (Type I), heNOS (25-54) and rheNOS (Type III). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 25-54.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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