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I1000 Interferon gamma, Recombinant, Mouse (IFNg) CAS:

Specifications
References
Grade
Highly Purified
Molecular Weight
15.6
EU Commodity Code
30021019
Shipping Temp
RT
Storage Temp
-20°C

Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors. Recombinant murine IFNg is a 15.6kD protein containing 134 amino acid residues.

Biological Activity
Determined by its ability to inhibit the proliferation of mouse WEHI-279 cells. ED50 is ≤0.2ng/ml, corresponding to a specific activity of ≥5 x 10e6units/mg.
Amino Acid Sequence
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA, HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Concentration
~1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0, 25mM sodium chloride. Reconstitute in ddH2O to 1mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
Gardner, J.L et al., (2007) Am J Physiol Gastrointest Liver Physiol 292:G1695-G170.
USBio References
US Biological application reference: Gardner, J.L et al., (2007) Am J Physiol Gastrointest Liver Physiol 292:G1695-G170.
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