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Interferon gamma, Recombinant, Mouse (IFNg)
Interferon gamma, Recombinant, Mouse (IFNg)
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| Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors. Recombinant murine IFNg is a 15.6kD protein containing 134 amino acid residues. | | | Catalog # | I1000 | | Biological Activity | The ED50 as determined by a cytopathic effect inhibition assay with murine NIH 3T3 cells challenged with VSV virus was 0.1ng/ml, corresponding to a specific activity of 1x10e7units/mg. For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1-10ng/ml. | | AA Sequence | MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 5-10mM sodium phosphate, pH 8.0. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Country of Origin | USA | | Molecular Weight | 15.6kD | | Source | E. coli | | Purity | 98% by SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug). | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0, 25mM sodium chloride. Reconstitute in sodium phosphate, pH 8.0 to 1mg/ml. Further dilutions should be made into RPMI + 10% FCS or 5% HSA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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