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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1890-05A
Applications	Suitable for use in Immunoblotting, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000 to 1:30,000 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT.
	Optimal dilution determined by the researcher.
Label	Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio	10-20 BAC molecules per Goat IgG molecule
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	1.0 mg/ml (UV280)
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA
(IgG and Protease free), 0.01% sodium azide. Biotin conjugated.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human IgA (alpha chain)
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, human IgA and human serum. No reaction was observed against other human heavy or light chain proteins
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.