Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to IgA » Anti -IgA, alpha (Biotin)

Anti -IgA, alpha (Biotin)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1890-05A
ApplicationsSuitable for use in Immunoblotting, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended DilutionsELISA: 1:10,000 to 1:30,000 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per Goat IgG molecule
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration1.0 mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA
(IgG and Protease free), 0.01% sodium azide. Biotin conjugated.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgA (alpha chain)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, human IgA and human serum. No reaction was observed against other human heavy or light chain proteins
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links