Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IF

Catalog #	I1890-05K
Applications	Suitable for use in Immunfluorescence and ELISA. Other applications have not been tested.
Recommended Dilutions	Immunofluorescence Microscopy: 1:1000-1:5000
	ELISA: 1:10,000-1:50,000
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	495nm
Emission Wavelength	528nm
Fluorochrome/Protein Ratio	9.4 moles FITC per mole of Goat IgG
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Rat
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC) (MW: 390 daltons). Reconstitute in 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography .
Immunogen	Rat IgA alpha heavy chain
Specificity	Recognizes rat IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgA and rat serum. Shows <1% crossreactivity in ELISA against other rat heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.