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You are here:Home » Antibodies » Abs to IgA » Anti -IgA, alpha (FITC)

Anti -IgA, alpha (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IF
Catalog #I1890-05K
ApplicationsSuitable for use in Immunfluorescence and FLISA. Other applications have not been tested.
Recommended DilutionsImmunofluorescence Microscopy: 1:1000-1:5000
FLISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength 528nm
Fluorochrome/Protein Ratio9.4 moles FITC per mole of Goat IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC) (MW: 390 daltons). Reconstitute in 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography .
ImmunogenRat IgA alpha heavy chain
SpecificityRecognizes rat IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgA and rat serum. Shows <1% crossreactivity in ELISA against other rat heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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