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You are here:Home » Antibodies » Abs to IgA » Anti -IgA (Biotin)

Anti -IgA (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1890-05L
ApplicationsSuitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:100,000, 1ug of Rat IgA in a standard capture using Streptavidin (HRP) and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA
(IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenRat IgA alpha heavy chain
SpecificityRecognizes rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgA and rat serum. Does not cross-react with other rat heavy or light chain proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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