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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1890-05L
Applications	Suitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000-1:100,000, 1ug of Rat IgA in a standard capture using Streptavidin (HRP) and ABTS (A0030) as a substrate for 30 minutes at RT.
	Western Blot: 1:2000-1:10,000
	Immunohistochemistry: 1:1000-1:5000
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Rat
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA
(IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Rat IgA alpha heavy chain
Specificity	Recognizes rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgA and rat serum. Does not cross-react with other rat heavy or light chain proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.