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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IF

Catalog #	I1890-05P
Applications	Suitable for use in Immunofluorescence, FLISA, and fluorescent Western Blot. Other applications have not been tested.
Recommended Dilutions	Immunofluorescence: 1:1000-1:5000
	FLISA: 1:10,000-1:50,000
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	495nm
Emission Wavelength	528nm
Fluorochrome/Protein Ratio	8.3 moles FITC per mole of Rabbit IgG.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgA alpha heavy chain
Specificity	Recognizes mouse IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgA and mouse serum. No reaction was observed against mouse IgG or mouse IgM.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.