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You are here:Home » Antibodies » Abs to IgA » Anti -IgA, alpha (Biotin)

Anti -IgA, alpha (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1890-05Q
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Other applications not tested.
Recommended DilutionELISA: 1:20,000-1:100,000
Western Blot:1:2000-1:10,000
Immunohistochemistry:1:1000-1:5000
Optimal dilutions to be determined by
the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10 mg/ml BSA, 0.1% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile 40-50% glycerol, dH2O.
PurityPurified by Immunoaffinity chromotography.
ImmunogenMouse IgA (alpha chain).
SpecificityRecognizes Mouse IgA (alpha chain). Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgA and Mouse Serum. No reaction was observed against other Mouse heavy or light chain proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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