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You are here:Home » Antibodies » Abs to IgA » Anti -IgA1,2

Anti -IgA1,2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
IgA is useful in the identi cation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid in ltrates indicates that the in ltrate is clonal and therefore malignant.
Catalog #I1890-11A
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraf n): 2-4ug/ml. Note: Staining of formalin/paraf n tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10–20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.334
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See I1890-11AX.
PurityPurified by Protein G affinity chromatography.
ImmunogenPuri ed human alpha heavy chain. Epitope: Third constant domain (CH3) of the alpha chain of IgA.
SpecificityRecognizes the alpha heavy chain of human immunoglobulins (50kD). Does not crossreact with gamma (IgG), mu (IgM), epsilon (IgE) or delta (IgD), heavy chains, T-cells, monocytes, granulocytes or erythrocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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