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You are here:Home » Antibodies » Abs to IgA » Anti -IgA, alpha (FITC)

Anti -IgA, alpha (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified FC IF
Catalog #I1890-17
ApplicationsSuitable for FLISA (Fluorescent), Immunomicroscopy, Flow Cytometry and Immunoflorescence. Other applications have not been tested.
Recommended DilutionsFLISA (Fluorescent): 1:10,000-1:50,000
Immunofluorescence: 1:1000-1:5000
Flow Cytometry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio2.1 moles FITC per mole of Goat IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography
ImmunogenHuman IgA (alpha chain)
SpecificityRecognizes human IgA (alpha chain). Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgA and human serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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