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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified FC IF

Catalog #	I1890-17
Applications	Suitable for FLISA, Immunomicroscopy, Flow Cytometry and Immunoflorescence. Other applications have not been tested.
Recommended Dilutions	FLISA: 1:10,000-1:50,000
	Immunofluorescence: 1:1000-1:5000
	Flow Cytometry: 1:500-1:2500
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	495nm
	Emission Wavelength: 528nm
Fluorochrome/Protein Ratio	2.1 moles FITC per mole of Goat IgG
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography
Immunogen	Human IgA (alpha chain)
Specificity	Recognizes human IgA (alpha chain). Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgA and human serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.