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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E IH

Catalog #	I1890-20C
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: (frozen) 1:10
	ELISA: 1:250 (as detecting antibody)
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents	I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
	I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Hybridoma	Spleen cells from immunized mice were fused with cells of the mouse P3X63-Ag8.653 cell line.
	Storage and Stability:
	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2251
Host	Mouse
Source	Porcine
Concentration	As reported
Form	Supplied as a liquid, 0.1% sodium azide.
Purity	Tissue culture supernatant.
Immunogen	Porcine IgA.
Specificity	Recognizes porcine Ig alpha chain. No crossreactivity with IgM and IgG.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.