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You are here:Home » Antibodies » Abs to IgA » Anti -IgA (Texas Red)

Anti -IgA (Texas Red)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
IgA is useful in the identi cation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid in ltrates indicates that the in ltrate is clonal and therefore malignant.
Catalog #I1890-21A
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000
Immunofluorescence Microscopy: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 596nm
Emission Wavelength 620nm
Fluorochrome/Protein Ratio3.0 moles Texas Red per mole of Rabbit IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Texas Red® conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red™ Sulfonyl Chloride (MW: 625 daltons). Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgA heavy chain
SpecificityRecognizes mouse IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse IgA and mouse serum. Specificity was confirmed by ELISA at <1% against other mouse heavy or light chain combinations.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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