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You are here:Home » Antibodies » Antibodies-IgA » Anti -Secretory Component (Free and Bound)

Anti -Secretory Component (Free and Bound)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Serum E
Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in human serum or other body fluids and to determine its concentration.
Catalog #I1890-31D
ApplicationsSuitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended DilutionImmunoelectrophoresis: 2ul plasma or equivalent against 120ul antiserum
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul antiserum in 3mm
diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter
peripheral wells.
Optimal dilutions to be determined by the researcher.
Precipitin Titer1:32; tested against pooled normal human milk in agar-block immunodiffusion titration.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgA
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.
PuritySerum (delipidated and heat-inactivated).
ImmunogenPurified free human secretory component isolated from pooled milk is used for immunization.
SpecificityRecognizes human secretory component. Binds to both bound SC (secretory IgA) and free SC.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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