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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Catalog #	I1890-32A
IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma, found at a concentration of 0.4 to 2.2mg/ml. It plays a very important role in the first specific defense against natural infection. Secretory IgA differs from serum IgA in that it contains two additional peptides	the secretory component and the J chain.
Applications	Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Frozen and Paraffin sections
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents	I1904-66C: IgG, F(ab')2, X-Adsorbed (HRP) Pab RbxMo
	I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo
Hybridoma	NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2259
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in TBS, 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Specificity	Recognizes human secretory-piece and secretory IgA. No reaction against myeloma IgA (native or reduced and alkylated). Negative against normal human IgM, myeloma IgM, normal human IgG; free kappa or gamma chains and J-chain. Typical pattern on frozen and paraffin embedded sections of intestinal and breast tissue.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.