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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified E

IgA is the predominant immunoglobulin class in body secretions, such as saliva, tears, bronchial secretions, nasal mucosal secretions, prostatic fluid, vaginal secretions, and mucous secretions of the small intestines. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. It is also found in small amounts in blood. Because it is resistant to degradation by enzymes, secretory IgA can survive in harsh environments such as the digestive and respiratory tracts, to provide protection against microbes that multiply in body secretions. IgA does not activate complement, and opsonises only weakly. Its heavy chains are of the type . It exists in two forms, IgA1 (90%) and IgA2 (10%): IgA1 is found in serum and made by bone marrow B cells. In IgA2, the heavy and light chains are not linked with disulfide but with noncovalent bonds. IgA2 is made by B cells located in the mucosae and has been found to secrete into colostrum, maternal milk, tears and saliva.
Catalog #	I1890-33
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	Direct ELISA: 1:20,000 dilution will yield >1.0 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5C52
Host	Mouse
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Purity	Purified by Ion Exchange chromatography.
Immunogen	Purified human secretory IgA
Specificity	Recognizes all human IgA subtypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.