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You are here:Home » Antibodies » Abs to IgA » Anti -IgA, Secretory Component, Free and Bound (sIgA, Secretory IgA)

Anti -IgA, Secretory Component, Free and Bound (sIgA, Secretory IgA)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Highly Purified E
Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. Secretory component also has an affinity for polymeric IgM.
Catalog #I1890-33F
ApplicationsSuitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended DilutionsELISA: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Titre 1:64 when tested against normal human milk in agar block titration.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~10mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified by DEAE chromatography.
ImmunogenPurified free human Secretory Component isolated from pooled milk.
SpecificityRecognizes human IgA Secretory Component. Reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal human milk, using a high electroendosmosis agar plate, free secretory component is precipitated in the alpha-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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