| IgA is the predominant immunoglobulin class in body secretions, such as saliva, tears, bronchial secretions, nasal mucosal secretions, prostatic fluid, vaginal secretions, and mucous secretions of the small intestines. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. It is also found in small amounts in blood. Because it is resistant to degradation by enzymes, secretory IgA can survive in harsh environments such as the digestive and respiratory tracts, to provide protection against microbes that multiply in body secretions. IgA does not activate complement, and opsonises only weakly. Its heavy chains are of the type . It exists in two forms, IgA1 (90%) and IgA2 (10%): IgA1 is found in serum and made by bone marrow B cells. |
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| Catalog # | I1890-65 |
| Applications | Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2260 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Reduced and alkylated human IgA |
| Specificity | Recognizes human immunoglobulin alpha heavy chains. No crossreactivity observed with IgG, IgM or free kappa and lambda light chains. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
