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You are here:Home » Antibodies » Abs to IgA » Anti -IgA, Heavy Chain

Anti -IgA, Heavy Chain

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified IH
The most common feature of plasmacytomas, and certain non-Hodgkin’s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Useful in the identification of leukemias, plasmacytomas, and certain non- Hodgkin’s lymphomas.
Catalog #I1890-65H
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:250-1:500 for 60 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide, before the addition of glycerol to 40%.
PurityPurified
ImmunogenIgA isolated from a pool of normal human sera.
Cellular LocalizationCytoplasmic, MW of antigen: 50kD
SpecificityRecognizes a protein of 50kD, identified as alpha heavy chain of human immunoglobulins. Reacts with both IgA1 and IgA2 isotypes. Does not crossreact with gamma (IgG), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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