Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 5ug/ml. Use 50ul to label 2 x 10e7 cells in100ul sample volume. Optimal dilution to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by affinity chromatography.
Specificity
Recognizes the epsilon chain of rat IgE. Does not cross-react with other classes of rat immunoglobulin.
References
1. Bazin, H., Querinjean, P., Beckers, A., Heremans, J.F. and Dessy, F. (1974). Immunology 26: 713-723.||||2. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Plateau, B. and Urbain, J. (1978). J. Immunol. 121: 2077