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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

Catalog #	I1900-01J
Applications	Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 5ug/ml. Use 50ul to label 2 x 10e7 cells in100ul sample volume.
	Optimal dilution to be determined by the researcher.
Hybridoma	Spleen cells from an immunized BALB/c mouse fused with mouse SP2/0 myeloma cells
Recommended Negative Controls	Mouse IgG1 Negative Control: FITC
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2277
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide and 50% glycerol. Labeled with FITC.
Purity	Purified by affinity chromatography.
Immunogen	Rat IgE myeloma proteins (IR162, IR1016, IR2 and IR410)
Specificity	Specific for the Epsilon chain of rat immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.