| IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion. But it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies; and the life-threatening respiratory distress of asthma and anaphylactic shock. |
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| Catalog # | I1900-11B |
| Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 1:100-1:500 coating |
| Western Blot: 1:1000-1:10,000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken (HRP) Rb x Ch |
| I1906-12 IgY, Chicken (FITC) Rb x Ch |
| I1906-14 IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Human IgE purified from myeloma plasma. |
| Specificity | Reacts with IgE by ELISA, IEP and gel diffusion analysis. Serum known to be very low in IgE shows no reaction with the anti IgE. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
