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You are here:Home » Antibodies » Abs to IgE » Anti -IgE

Anti -IgE

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B Gel
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion. But it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies; and the life-threatening respiratory distress of asthma and anaphylactic shock.
Catalog #I1900-11B
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:100-1:500 coating
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgE purified from myeloma plasma.
SpecificityReacts with IgE by ELISA, IEP and gel diffusion analysis. Serum known to be very low in IgE shows no reaction with the anti IgE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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