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You are here:Home » Antibodies » Abs to IgE Conjugates » Anti -IgE (HRP)

Anti -IgE (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #I1900-30A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Affinity Constant4 x 10e9 M-1
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2277
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
PurityPurified by affinity chromatography.
ImmunogenRat IgE myeloma protein (IR162, IR410).
SpecificityRecognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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