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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Catalog #	I1900-30A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 0.5ug/ml
	Optimal dilutions to be determined by the researcher.
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Affinity Constant	4 x 10e9 M-1
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2277
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Purity	Purified by affinity chromatography.
Immunogen	Rat IgE myeloma protein (IR162, IR410).
Specificity	Recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.