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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IH

Catalog #	I1900-30C
Applications	Suitable for use in Immunohistochemistry, ELISA and
	Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry : 1:500-1:2500
	ELISA: 1:5000-1:50,000. Assayed against 1ug of Rat IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT.
	Western Blot: 1:1000-1:10,000
	Optimal working dilutions to be determined by researcher.
Label Molecular Weight	40,000
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Rat
Concentration	~2mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Rat IgG whole molecule
Specificity	Recognizes rat IgE. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum, rat IgG and rat serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.