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You are here:Home » Antibodies » Antibodies-IgE Conjugates » Anti -IgE (epsilon Chain) (FITC)

Anti -IgE (epsilon Chain) (FITC)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified IH IC FC IF
Catalog #I1900-42E5
ApplicationsSuitable for use in FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Optimal dilutions to be determined by the researcher.
Absorption 494nm
Emission 518nm
FITC/Protein Ratio 7 Moles FITC per Mole Antibody
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
PurityPurified by affinity chromatography. 95% by SDS-PAGE.
ImmunogenPurified human IgE, epsilon Chain
SpecificityRecognizes epsilon chains on human IgE based on IEP. No reactivity is observed with light chains on human immunoglobulins and on non-immunoglobulin human serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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