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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-01H
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoperxoidase Electron Microscopy. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:100,000
Immunohistochemistry: 1:500-1:5000
Western Blot: 1:500-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceBovine
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 0.01% gentamicin sulfate, 15mg/ml BSA (IgG and Protease free), 40% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaf nity chromatography.
ImmunogenBovine IgG F(c) fragment
SpecificityRecognizes bovine IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-goat serum, bovine IgG, bovine IgG F(c) and bovine serum. No reaction was observed against bovine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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