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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (FITC)

Anti -IgG, Fc (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1903-01W
ApplicationsSuitable for use in Immunofluorescence and ELISA. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
LabelFluorescein isothiocyanate (FITC) MW:390D
Absorption Wavelength 495nm
Emission Wavelength 528nm
Fluorochrome/Protein Ratio3.6 moles FITC per mole of Rabbit IgG.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol.
PurityPurified by immunoaf nity chromatography.
ImmunogenBovine IgG F(c) fragment
SpecificityRecognizes bovine IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti fluorescein, anti rabbit serum, bovine IgG, bovine IgGFc and bovine serum. No reaction against bovine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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