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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1903-01Y
Label Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio 10–20 BAC molecules per Rabbit IgG molecule
Applications Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2,000-1:15,000
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~1mg/ml (UV280)
FormSupplied as a sterile-filtered liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by immunoaf nity chromatography.
ImmunogenBovine IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum. No reaction was observed against Bovine IgG F(ab’)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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