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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1903-03E
Label Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio 1020 BAC molecules per Rabbit IgG molecule
Applications Suitable for use in ELISA, Western Blot and Immunohistochemistry.. Other applications not tested.
Recommended DilutionELISA: 1:50,000 to 1:200,000
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceFeline
Concentration~2mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by immunoaf nity chromatography.
ImmunogenFeline IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Feline IgG, Feline IgG F(c) and Feline Serum. No reaction was observed against Feline IgG F(ab)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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