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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (FITC)

Anti -IgG, Fc (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1903-04H
Label Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength 495 nm
Emission Wavelength 528 nm
Fluorochrome/Protein Ratio 2.8 moles FITC per mole of Rabbit IgG
Applications Suitable for use in immunofluorescence and ELISA. Other applications have not been tested.
Recommended Dilution Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGuinea pig
Concentration~2mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by immunoaf nity chromatography.
ImmunogenGuinea Pig IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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