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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (TRITC)

Anti -IgG, Fc (TRITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
Catalog #I1903-04J
Label Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength 550 nm
Emission Wavelength 570 nm
Fluorochrome/Protein Ratio 2.0 moles TRITC per mole of Rabbit IgG
Applications Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGuinea pig
Concentration~2mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Labeled with TRITC.
PurityPurified by immunoaf nity chromatography.
ImmunogenGuinea Pig IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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