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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, F(abí)2 (Biotin)

Anti -IgG, F(abí)2 (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-05D
ApplicationsSuitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy Other applications not tested.
Recommended DilutionELISA: 1:20,000 to 1:100,000. 1.0 ug of Cat IgG was used in a standard sandwich ELISA with Streptavidin (HRP) (S7973-97A) and ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (A0030) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio 10-20 BAC molecules per Goat IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceFeline
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
PurityPurified by immunoaffinity chromatography.
ImmunogenFeline IgG F(abí)2 fragment.
SpecificityAssay by IEP resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, feline IgG, feline IgG F(abí)2 and feline Serum. No reaction was observed against feline IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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