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You are here:Home » Antibodies » Abs to IgG IgA IgM » Anti -IgG, IgA, IgM, H&L (TRITC)

Anti -IgG, IgA, IgM, H&L (TRITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
Catalog #I1903-10E
Label Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength 550 nm
Emission Wavelength 570 nm
Fluorochrome/Protein Ratio 2.6 moles TRITC per mole of Goat IgG
Applications Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended Dilution Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Labeled with TRITC.
PurityPurified by immunoaf nity chromatography.
ImmunogenRat IgG whole molecule
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA, Rat IgG and Rat IgM. This reagent is suitable for the detection of all rat immunoglobulin subclases, isotypes and chain combinations.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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