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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1903-10H
Applications	Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000-1:50,000
	Immunofluorescence: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	550nm
Emission Wavelength	570nm
Fluorochrome/Protein Ratio	2.9 moles Rhodamine (TRITC) per mole of Rabbit IgG
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Rhodamine labeled product is light sensitive.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~1mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Lableled with Rhodamine (TRITC).
Purity	Purified by immunoaf nity chromatography.
Immunogen	Rat IgG F(c) fragment
Specificity	Recognizes rat IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, rat IgG, rat IgG F(c) and rat serum. No reaction was observed against rat IgG F(ab’)2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.