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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (TRITC)

Anti -IgG, Fc (TRITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1903-10H
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:50,000
Immunofluorescence: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength550nm
Emission Wavelength570nm
Fluorochrome/Protein Ratio2.9 moles Rhodamine (TRITC) per mole of Rabbit IgG
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Rhodamine labeled product is light sensitive.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Lableled with Rhodamine (TRITC).
PurityPurified by immunoaf nity chromatography.
ImmunogenRat IgG F(c) fragment
SpecificityRecognizes rat IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, rat IgG, rat IgG F(c) and rat serum. No reaction was observed against rat IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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